Purification, Partial Characterization and Immobilization of a Mannose-Specific Lectin from Seeds of Dioclea lasiophylla Mart.
نویسندگان
چکیده
منابع مشابه
Purification, partial characterization and immobilization of a mannose-specific lectin from seeds of Dioclea lasiophylla mart.
Lectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-D-mannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, ...
متن کاملPurification, partial characterization, and CNBr-sepharose immobilization of a vasorelaxant glucose/mannose lectin from Canavalia virosa seeds.
A novel mannose/glucose-binding lectin from Canavalia virosa (designated as ConV) has been purified from seeds of C. virosa by affinity chromatography on a mannose-Sepharose 4B column. ConV strongly agglutinates rabbit erythrocytes and was inhibited by monosaccharides (D-mannose, D-glucose, and α-methyl-D-mannoside) and glycoproteins (ovalbumin and fetuin). SDS-PAGE revealed three bands corresp...
متن کاملPurification and Characterization of a Lectin from VEstaria floribunda Seeds
A lectin from Wisturi~ floribunda seeds which specifically binds to N-acetyl-bgalactosamine was purified to homogeneity as judged by polyacrylamide gel electrophoresis. Its molecular weight was estimated to be 68,000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. It dissociated into subunits on reduction with 2-mercaptoethanol with concomitant loss of hemagglut...
متن کاملIsolation, purification and partial physicochemical characterization of a lectin in Andira pisonis Mart seed
Lectins are ubiquitous proteins in nature, with nonimmune origin, which have at least one non-catalytic domain that binds carbohydrates specifically and reversibly. They can be found in vegetables leaves, stems and seeds. The Dalbergieae tribe has lectins which have specificity for different carbohydrates and also have several biological activities such as induction of rat paw edema, release of...
متن کاملCrystallization and characterization of an inflammatory lectin purified from the seeds of Dioclea wilsonii.
DwL, a lectin extracted from the seeds of Dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and ABO erythrocytes, inhibited by glucose and mannose. DwL was purified by affinity chromatography on a Sephadex G-50 column and ion exchange chromatography on a HiTrap SP XL column. SDS-PAGE revealed three electrophoretic bands corresponding to the α (25,634 ± 2 Da...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecules
سال: 2013
ISSN: 1420-3049
DOI: 10.3390/molecules180910857